Our guide highlights the use of Fluorescent Probes and Dyes in research and provides background information on various imaging techniques. & Earn an Amazon Gift Card, OC(C(S(=O)(O[Na])=O)=CC4=C3C=CC(S(=O)(O[Na])=O)=C4)=C3/N=N/C(C=C2)=C(S(=O)(O[Na])=O)C=C2/N=N/C1=CC=C(S(=O)(O[Na])=O)C=C1, Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution), TSA Reagents for Enhancing IHC, ICC & FISH Signals. Following protein electrotransfer, rinse membrane briefly in ddH2O to remove any detergent that may inhibit staining. trichrome stain cardiac tissues modified examples masson urmc rochester edu TBS/BSA). If incorrect, please enter your country/region into the box below, to view site information related to your country/region. Why not submit a protein staining l1 system sensitivity superior ng genscript protein staining l1 system sensitivity superior ng genscript Any Customer's terms and conditions that are in Average Rating: 4.3 (Based on 3 Reviews. Changing to another country might result in loss of shopping cart. 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. Stain used to detect protein bands after Western blotting, 3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt, OC(C(S(=O)(O[Na])=O)=CC4=C3C=CC(S(=O)(O[Na])=O)=C4)=C3/N=N/C(C=C2)=C(S(=O)(O[Na])=O)C=C2/N=N/C1=CC=C(S(=O)(O[Na])=O)C=C1, Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution). Custom Services for Cell & Gene Therapies, Good Manufacturing Practice (GMP) Antibody Services. Any use of Product for diagnostic, *When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online). Do not dilute or aliquot. Bacterial peptidoglycan derivative; suitable for 'click'-conjugation to fluorescent dyes. To get a clear background, wash the membrane quickly in PBS after staining2. Take a look at our BETA site and see what weve done so far. Ponceau S Solution (ab270042) is a ready-to-use reversible stain which allows you to check the efficiency of electrophoretic transfer of SDS-denatured proteins to nitrocellulose and PVDF membranes. ab270042 has not yet been referenced specifically in any publications. The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE copyright notices or markings, (d) use the Products solely in accordance with Agonists, activators, antagonists and inhibitors, Anti-CYP2C9 antibody [EPR7340] (ab150364). This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. industry. Access advice and support for any research roadblock, Full event breakdown with abstracts, speakers, registration and more, Explore the power of knock-out cell lines for your research. Ponceau S Staining Solution is a stain used to detect protein bands after Western blotting. The stain on the membrane can be washed off with PBS or TBS so that it can be further incubated with the primary antibody for immunodetection. This ponceau S solution was used to stain total protein that had been applied to PVDF membrane using during a slot blot experiment. documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or Tocris Bioscience is the leading supplier of novel and exclusive Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. For batch specific data refer to the Certificate of Analysis. 5. If you know of a relevant reference for Ponceau S Staining Solution, please let us know. Do you know of a great paper that uses Ponceau S Staining Solution from Tocris? As little as 250 ng protein can be rapidly visualized with Ponceau S stain. wse staining This reagent stains protein bands very quickly (less than 1 min) and has the big advantage to be easily destained after a quick wash step that can be made with water or PBS. Currently there are no citations for Ponceau S Staining Solution. ProteinSimple branded instruments are available to quote. 3. The membrane was stained with Ponceau S solution for less than a minute to detect protein bands and confirm successful sample run and transfer (lane 2-6) or not (lane 1). Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available Which Brands are Currently Available on biotechne.com? This enables one to incubate the different membrane sections with various primary antibodies. masson trichrome staining x10 special By continuing to use this website you acknowledge this and agree to our cookie policy. structure or technology of the Products, or use the Products for the purpose of developing any products or services that would This product is manufactured by Expedeon, an Abcam company. There are no For batch specific data refer to the Certificate of Analysis. There are no reviews for Ponceau S Staining Solution (NB5225). by the FDA or other regulatory foreign or domestic entity, for any purpose. Citations are publications that use Tocris products. References are publications that support the biological activity of the product. This product is stable for 12 months. Ponceau S Staining Solution Support Products, View other related Ponceau S Staining Solution Support Products, Sander H, Wallace S, Plouse R et al. We have publications tested in 1 application: WB. life science research with over 30 years' experience in the Please contact us to place your order, or try again later. decide on the best products for their research. $25/18/15/$25CAN/75 Yuan/1250 Yen for a review with an image, $10/7/6/$10 CAD/70 Yuan/1110 Yen for a review without an image. Minneapolis, MN 55413 All Rights Reserved. Be the first to Please let us know. It is also used in our lab to detect protein degradation or any other abnormalities like overloading (as shown in the figure, the line #9 contains a protein sample degrade). Tocris products are intended for laboratory research use only, unless stated otherwise. To download a Certificate of Analysis, please select a batch below. This enables one to incubate the different membrane sections with various primary antibodies. No. All rights reserved. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest. Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed Tocris is a Bio-Techne brand. 5225) to confirm successful transfer of proteins from the gel onto nitrocellulose membranes during the transfer step of western blot analysis. Lanes 1 to 8 Thermo Scientific Page Ruler Plus, lane 2 E.coli lysate, lane 3 Chicken lysate, lane 4 2 g BSA, lane 5 1 g BSA, lane 6 500 ng BSA, lane 7 250 ng BSA. Your browser does not have JavaScript enabled and some parts of this website will not work without it. This product is for research use only and is not approved for use in humans or in clinical diagnosis. Copyright 2022 R&D Systems, Inc. All Rights Reserved. Please let us know so that we can cite the reference in this datasheet. any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized Publishing research using ab270042? It was previously called Ponceau S Solution - 1L. ponceau staining solution tocris stain cas services used by Customer in connection with the Products. the vial label and CoA (available online). Would you like to visit your country specific website? The membrane was stained with Ponceau S solution for less than a minute to detect protein bands and confirm successful sample run and transfer (lane 2-6) or not (lane 1). Copyright 2022 Bio-Techne. 30 ug of protein was loaded per well and image was taken using a LiCor Odyssey imager The staining worked but the signal was not as strong as other Ponceau S stains I have used before. For batch specific data refer to the Certificate of Analysis. The technical data provided above is for guidance only. Customer shall not use any Product for any diagnostic Your If desired, mark proteins with waterproof ink or a pencil. Protein samples were resolved in NuPAGE 412% Bis-Tris gel (Invitrogen) and transferred to 0.2 m PVDF membrane. Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments. We offer a 100% guarantee on all our products. The intensity of staining corresponds to the amount of protein transferred. Store at room temperature. Expedeon product code 5999-1L is the same as the1000 mL size of this product. review will help other researchers Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms Products sold or licensed by CST compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or Moreover, it is also useful to select the membrane area to be cut after transfer, based on the migration pattern of the protein standards (line #1). *Please note that Tocris will only send literature to established scientific business / institute addresses. Please note: All products are "FOR RESEARCH USE ONLY. 6. Tocris products are intended for laboratory research use only, unless stated otherwise. Store at Room Temperature. are provided for Customer as the end-user and solely for research and development uses. 1998-2022 Abcam plc. requires a separate license from CST. commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying The technical data provided above is for guidance only. There are no specific FAQs related to this product. Ponceau S Staining Solution is a stain used to detect protein bands after Western blotting. Get the latest news, product updates, and promotions: Soluble in 5% acetic acid/water (supplied as a 0.1% w/v solution). Products in stock will be shipped in 1-3 business days unless specified. CST Product Terms of Sale and any applicable The reviewer received a reward from Abcams Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions. 1. For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. Following membrane transfer, users can directly incubate the blotting membrane (PVDF or nitrocellulose) in ~15ml of Ponceau S solution without the need for any wash steps. You can now submit reviews for your favorite Tocris products. R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through biotechne.com. There are currently no reviews for this product. The technical data provided above is for guidance only. vial. No. We use Ponceau S (Cat. This product guide provides a background to the use of Fluorescent Probes and Dyes, as well as a comprehensive list of our. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. All other trademarks are the property of their respective owners. Get the latest news, product updates, and promotions: Stain to detect protein bands and confirm sucessful transfer prior to antibody incubation.. Best staining for tracking protein transfer in western blot. Tocris products are intended for laboratory research use only, unless stated otherwise. Chemical Name: 3-Hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid tetrasodium salt. Read our general, There are no specific blogs for Ponceau S Staining Solution, but you can. No product specific FAQs exist for this product, however you may. Calculate the dilution required to prepare a stock solution. Constituents: 0.1% Ponceau S, 0.96% Acetic acid. View all citations for the Staining Kits and Reagents platform. Mar 23 2019 [PMID: 30914243] (WB), Review with no image -- $10/7/6/$10 CAD/70 Yuan/1110 Yen, Review with an image -- $25/18/15/$25 CAD/150 Yuan/2500 Yen. prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Wash membrane in ddH2O until distinct reddish-pink protein bands are visible (1-5 min). Bio-Techne uses cookies to provide you with a great website experience. trichrome masson diagnostics ral solutions kit 1. trichrome masson diagnostics ral solutions kit 1. ), $50/35/30/$50CAN/300 Yuan/5000 Yen for first to review with an image, $25/18/15/$25CAN/75 Yuan/1250 Yen for a review with an image, $10/7/6/$10 CAD/70 Yuan/1110 Yen for a review without an image. Note: Mouseover a species abbreviation on the product page to display the fullname. wse staining Keywords: Ponceau S Staining Solution, Ponceau S Staining Solution supplier, Ponceau, S, Staining, Solution, stain, detect, protein, bands, western, blotting, Labels, and, Stains, 5225, Tocris Bioscience. All transferred protein slowly becomes visible as red bands on the membrane, with highest intensity of staining reached within 5 minutes incubation with Ponceau S. Rinsing the membrane with water after Ponceau S staining can help reduce background staining on the blotting membrane. Calculate the mass, volume, or concentration required for a solution. To get a clear background, wash the membrane quickly in PBS after staining2. Fluorescent Probes and Dyes Product Guide, Citations for Ponceau S Staining Solution, GMP & Ancillary Material Grade Small Molecules, Certificate of Analysis / Product Datasheet, Submit a Review Alternatively, a photograph of the stained membrane can also be taken. USA, TEL 612 379 2956 2. tools for Azide-containing monosaccharide building block; used as glycoprotein, glycolipid and glycoRNA probe, Amine reactive biotinylation reagent, cell permeable. trichrome stain cardiac tissues modified examples masson urmc rochester edu Ponceau red stain is not stable and will disappear if you destain longer.3.